Background

This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

Images

	Western blotting analysis using anti-kinesin family member 4A antibody (Cat#61161). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-kinesin family member 4A antibody (Cat#61161, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-kinesin family member 4A antibody (Cat#61161). Kinesin family member 4A expression in wild type (WT) and kinesin family member 4A (KIF4A) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-kinesin family member 4A antibody (Cat#61161, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of kinesin family member 4A expression in HAP-1 cells using anti-kinesin family member 4A antibody (Cat#61161, 1:2,000). Green, isotype control; red, kinesin family member 4A.
	Immunocytochemical staining of HAP1 cells with anti-kinesin family member 4A antibody (Cat#61161, 1:1,000). Nuclei were stained blue with DAPI; Kinesin family member 4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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