Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	interferon regulatory factor 6
Gene Symbol:	IRF6
Synonyms:	LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1
Gene ID:	3664
UniProt ID:	O14896
Clone ID:	24GB6015
Immunogen:	A synthesized peptide derived from human IRF6
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-interferon regulatory factor 6 antibody (Cat#61158). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-interferon regulatory factor 6 antibody (Cat#61158). Interferon regulatory factor 6 expression in wild type (WT) and interferon regulatory factor 6 (IRF6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of interferon regulatory factor 6 expression in C2C12 cells using Interferon regulatory factor 6 antibody (Cat#61158, 1:2,000). Green, isotype control; red, interferon regulatory factor 6.
	Immunocytochemical staining of HepG2 cells with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:1,000). Nuclei were stained blue with DAPI; Interferon regulatory factor 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.