Description

Reactivity:	Human
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	growth factor receptor bound protein 7
Gene Symbol:	GRB7
Synonyms:	GRB7; Growth Factor Receptor Bound Protein 7; Growth Factor Receptor-Bound Protein 7; Epidermal Growth Factor Receptor GRB-7; GRB7 Adapter Protein; B47
Gene ID:	2886
UniProt ID:	Q14451
Clone ID:	24GB6000
Immunogen:	A synthesized peptide derived from human GRB7
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-anti-growth factor receptor bound protein 7 antibody (Cat#61153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-growth factor receptor bound protein 7 antibody (Cat#61153). Growth factor receptor bound protein 7 expression in wild type (WT) and growth factor receptor bound protein 7 (GRB7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of growth factor receptor bound protein 7 expression in HepG2 cells using growth factor receptor bound protein 7 antibody (Cat#61153, 1:2,000). Green, isotype control; red, growth factor receptor bound protein 7.
	Immunocytochemical staining of HepG2 cells with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:1,000). Nuclei were stained blue with DAPI; Growth factor receptor bound protein 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.