Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC, IC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	destrin, actin depolymerizing factor
Gene Symbol:	DSTN
Synonyms:	ADF; ACTDP; HEL32; bA462D18.2
Gene ID:	11034
UniProt ID:	P60981
Clone ID:	24GB5920
Immunogen:	A synthesized peptide derived from human Destrin
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene.

Images

	Western blotting analysis using anti-DSTN antibody (Cat#61122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DSTN antibody (Cat#61122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-DSTN antibody (Cat#61122). DSTN expression in wild type (WT) and DSTN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DSTN antibody (Cat#61122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of DSTN expression in H9c2 cells using DSTN antibody (Cat#61122, 1:2,000). Green, isotype control; red, DSTN.
	Immunocytochemical staining of H9C2 cells with anti-DSTN antibody (Cat#61122, 1:1,000). Nuclei were stained blue with DAPI; DSTN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.