Description

Based on our proprietary polymer synthesis technology, PeneFect™ Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. PeneFect™ reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis , leading to much less cytotoxicity. PeneFect™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.

Key Features

1. Bio-degradable after endocytosis

2. Exceptional high titers of virus production

3. Equally good for very long DNAs (>89 kb)

4. Equally good for both single DNA transfection and multi DNA co-transfection

5. High levels of recombinant protein production

6. Simple & robust transfection procedure)

7. Very affordable

Components

1. PeneFect™ Transfection Reagent: 4 ml

Storage

Store at 4°C.

Other Size

Product Name	Cat. #	Price	View
PeneFect™ Transfection Reagent (1 ml)	M0001-01	$149

 

Case Study

A comparison showing transfection efficiency of PeneFect™ reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells. 
HEK-293T cells were transfected with GFP vector (pEGFP-N3) by PeneFect™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

A comparison showing transfection efficiency of PeneFect™ reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells. 
HepG2 cells were transfected with GFP vector (pEGFP-N3) by PeneFect™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.