Description

The TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material which the silica membrane is made of is unique, highly efficient and highly specific. This kit is designed for purification of up to 24 μg plasmid DNA from 1-4 ml overnight cultures of E.coli. The plasmid DNA purified by this kit can be applied in various regular experiments, such as enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro transcription and the transfection of the common sub-culture cells, etc.

 

Key Features

• Fast: Simple procedure with minimal steps to purify high pure plasmid DNA within only 8 min.

• High efficiency: Could purify more than 85% plasmid DNA from E.coli.

• The color indicator TIANRed was supplied to control and remind every process during the experiment to ensure the high efficiency of plasmid purification.

 

Specifications

Plasmid Type	Bacterial Culture Volume	Yield	Plasmids
Low Copy	1-5 ml	3-12 μg	pBR322, pACYC, pSC101, SuperCos, pWE15
High Copy	1-5 ml	6-30 μg	pTZ, pUC, pBS, pGM-T

 

Components

1. RNase A (10 mg/ml): 600 μl
2. Buffer P1: 60 ml
3. Buffer P2: 60 ml
4. Buffer P5: 80 ml
5. Buffer PWT: 50 ml
6. Buffer TB: 30 ml
7. TIANRed: 300 μl
8. Spin Columns CP3: 200
9. Collection Tubes 2 ml: 200

 

Storage

Store at room temperature.

 

Case Study

Comparison between TIANprep Rapid Mini Plasmid Kit and Supplier A (general plasmidDNA purification kit) by purifying the same plasmid at the same time. Agarose gel electrophoresis shows that TIANprep Rapid Mini Plasmid Kit could obtain same yield of plasmid DNA as that by the general plasmid purification kit within 8 min.