Description

The TGuide Smart Endofree Mini+ Plasmid Kit 1 uses magnetic beads with unique separation properties and a unique buffer system to isolate and purify 2~100 µg of high quality plasmid DNA from 5~20 ml of bacterial broth. The uniquely embedded magnetic beads have a strong affinity for plasmid DNA under certain conditions, and when the conditions are changed, the magnetic beads release the adsorbed plasmid DNA, which can achieve the purpose of rapid isolation and purification of plasmid DNA, and can maximize the removal of proteins and other impurities, thus ensuring the purity of the extracted plasmid. Plasmid DNA extracted with this kit can be used in a variety of molecular biology experiments, such as enzymatic cleavage, sequencing, library screening, ligation and transformation.

Key Features

• Simple and fast: Ultra-pure plasmid DNA can be obtained within 45 min.

• Widely applicable: Suitable for both high copy plasmids and low copy plasmids.

• Safe and non-toxic: No toxic organic reagents such as phenol/chloroform are required.

• High purity: The plasmid DNA obtained is of high purity and can be directly used in downstream experiments such as enzyme digestion and sequencing..

Components

1. RNase A (100 mg/ml): 300 μl
2. Buffer P1: 30 ml
3. Buffer DP2: 15 ml
4. Buffer DP4: 15 ml
5. Endotoxin Removal Buffer ER: 4 ml
6. Plasmid DNA Reagents: 48 Pcs
7. TGuide Smart Tip Comb: 12 Pcs

Storage

The kit can be stored in dry conditions at room temperature (15~30°C) for 12 months.

Super easy to use

No extra pipetting work

The elution volume can be adjusted as required.

The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.

Preloaded reagents and matched disposable consumables.

The chemical handling is eliminated to the greatest extent to ensure the operation safety.