Description

LiTaq™ Ultra HiFi DNA Polymerase is a next-generation super-fidelity DNA polymerase engineered through directed evolution of LiTaq™ High Fidelity Polymerase. It delivers ultra-high fidelity (error rate 100× lower than conventional Taq polymerase) and fast amplification (5–10 sec/kb). Incorporating advanced hot-start technology and unique stabilizing factors, this high fidelity polymerase significantly enhances amplification specificity, success rate, and product yield across a wide range of templates. The HiFi Taq polymerase produces blunt-ended amplicons, making it ideal for downstream applications such as cloning with the LiClone™ Ultra One Step Cloning Kit (Cat. #: M0011).

Key Features

• Exceptional Fidelity: Over 100× higher accuracy than standard Taq DNA Polymerase.

• Fast Amplification: Extends at speeds up to 10 sec/kb.

• Broad Applicability: Ideal for routine PCR, long amplicons, and difficult templates.

• High Performance: Achieves robust amplification of fragments up to 20 kb with minimal optimization.

• Enhanced Yield: Generates high product yields even with low enzyme amounts.

• GC-Rich Capability: No buffer adjustment needed for GC-rich targets.

• Superior Specificity: Outperforms most commercially available polymerases.

Applications

• High fidelity PCR

• High yield and fast PCR

• Blunt end cloning

• Complex templates

• GC-rich PCR

• Site-directed mutagenesis

Component

1. LiTaq™ Ultra HiFi DNA Polymerase: 500 U

Storage

Store at -20°C.

Case Study

Samples: Human Genomic DNA & cDNA, Blood Samples, Mouse Tail Lysate

Product: LiTaq™ Ultra HiFi DNA Polymerase

Competitors: Takara PrimeStar Max, Thermo Platinum Pfx

PCR reaction setup for amplification: • 2× LiTaq HiFi Buffer: 25 µl • dNTP Mix (10 mM each ): 25 µl • F1: 2 µl • R1 : 2 µl • Human gDNA : 1 µl • ddH2O: 18 µl

PCR cycling for amplification: 1. 95°C: 3 min 2. 35 x 1. Denaturation: 15 s at 95°C 2. Annealing: 15 s at 63°C 3. Extension: 30 sec/kb at 72°C 3. Final extension: 5 min at 72°C

From the above results, it can be concluded that LiTaq™ High Fidelity Taq Polymerase is suitable for the amplification of a variety of difficult templates. Under high GC, low template concentration and high inhibitor conditions, LiTaq™ DNA polymerase invariantly performed better than other polymerases. Although direct amplification of lysates is common practice for shorter fragments, amplification of longer fragments is more consistent with LiTaq™ DNA polymerase.