Description

The LiQuant™ NGS dsDNA Assay Kit is expertly crafted for the straightforward and precise quantification of double-stranded DNA (dsDNA). This kit stands out for its high selectivity for dsDNA over RNA and provides superior stability, a broad linear dynamic range, and heightened sensitivity compared to conventional DNA quantitation methods.

Featuring an “Add-and-Read” format, the kit streamlines the DNA quantitation process and accelerates your workflow. It is particularly suited for DNA quantitation in Next Generation Sequencing (NGS) library preparations. The assay’s robust design ensures tolerance to common contaminants, such as proteins, salts, solvents, detergents, or free nucleotides, which can often complicate quantitation efforts.

Moreover, the assay’s flexibility allows for adaptation to various experimental formats, including microplates, tubes, or cuvettes, making it a versatile choice for different laboratory settings and high-throughput sequencing applications.

Key Features

• Higher sensitivity: 100 times more sensitive than NanoDrop.

• Broad dynamic range: over four orders of magnitude.

• More accuracy: unaffected by the presence of proteins, free nucleotides.

• Easy to use: mix-and-read format.

• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.

Applications

dsDNA quantitation of DNA samples for Next Gen Sequencing

Specifications

1. Sample Type: dsDNA

2. Platform: Fluorescence plate reader or fluoromete

3. Linear Range: 0.2-100 ng dsDNA

Components

1. LiQuant™ 1× dsDNA HS Reagent: 40 ml
2. dsDNA Standard #1: 0.4 ml
3. dsDNA Standard #2: 0.4 ml

Storage

Store at 2-8°C and protect from light.

Case Study

Left: Quantitation of dsDNA with LiQuant™ NGS dsDNA Assay Kit using fluorescence plate reader. Right: Quantitation of dsDNA with LiQuant™ NGS dsDNA Assay Kit using Qubit® Fluorometer.