Description

LiGreen™ Red DNA Gel Stain is a sensitive, and stable fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for detection of dsDNA, ssDNA or RNA in agarose and polyacrylamide gels. This single stain gives more sensitive detection of dsDNA, ssDNA and RNA than EtBr. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. LiGreen™ Red has similar excitation and emission spectra with EtBr, and is compatible with EtBr imaging system.

Gel staining with LiGreen™ Red is compatible with downstream applications such as gel extraction and cloning. RedView is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Key Features

• Safer than EB. Shown by Ames test and other tests to be non-mutagentic and non-cytotoxic.

• Stable at room temperature.

• Compatible with standard blue LED and UV transilluminators.

• Use with blue light reduces mutagenesis for gel excision and subsequent cloning.

Component

LiGreen™ Red DNA Gel Stain (10,000× in DMSO): 500 μl

Storage

Store at 2-25°C and protect from light.