Background

Enables SNAP receptor activity; SNARE binding activity; and protein phosphatase binding activity. Involved in several processes, including autophagosome membrane docking; endoplasmic reticulum to Golgi vesicle-mediated transport; and endoplasmic reticulum-Golgi intermediate compartment organization. Acts upstream of or within protein localization to phagophore assembly site. Located in several cellular components, including autophagosome membrane; endoplasmic reticulum-Golgi intermediate compartment; and mitochondria-associated endoplasmic reticulum membrane contact site. Part of SNARE complex.

Images

	Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Syntaxin 17 expression in wild-type (WT) and syntaxin 17 (STX17) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Syntaxin 17 expression in HepG2 cells using anti-Syntaxin 17 antibody (Cat#71159, 1:2,000). Green, isotype control; red, Syntaxin 17.
	Immunocytochemical staining of HepG2 cells with anti-Syntaxin 17 antibody (Cat#71159, 1:1,000). Nuclei were stained blue with DAPI; Syntaxin 17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.