Background

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-protein regulator of cytokinesis 1 antibody (Cat#71220). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein regulator of cytokinesis 1 antibody (Cat#71220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-protein regulator of cytokinesis 1 antibody (Cat#71220). Protein regulator of cytokinesis 1 expression in wild-type (WT) and protein regulator of cytokinesis 1 (PRC1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein regulator of cytokinesis 1 antibody (Cat#71220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Protein regulator of cytokinesis 1 expression in HepG2 cells using anti-Protein regulator of cytokinesis 1 antibody (Cat#71220, 1:2,000). Green, isotype control; red, Protein regulator of cytokinesis 1.
	Immunocytochemical staining of HepG2 cells with anti-Protein regulator of cytokinesis 1 antibody (Cat#71220, 1:1,000). Nuclei were stained blue with DAPI; Protein regulator of cytokinesis 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.