Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127).