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KO-Validated ALDH1A3 Mouse mAb (20 μl)

$149.00

Cat. #: 71254

Description

Reactivity: Human
Applications: WB
Host Species: Mouse
Isotype: IgG1
Clonality: Monoclonal antibody
Gene Name: aldehyde dehydrogenase 1 family member A3
Gene Symbol: ALDH1A3
Synonyms: ALDH6; MCOP8; RALDH3; ALDH1A6
Gene ID: 220
UniProt ID: P47895
Clone ID: 25GB3805
Immunogen: Recombinant protein of human ALDH1A3
Dilution: WB 1:500-1:2,500
Purification Method: Affinity purified
Concentration: Lot dependent
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.

 


 

Background

The gene ALDH1A3 encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

 


 

Images

ALDH1A3 antibody Western blotting analysis using anti-ALDH1A3 antibody (Cat#71254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALDH1A3 antibody (Cat#71254, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
71254 WB KO 1 Western blotting analysis using anti-ALDH1A3 antibody (Cat#71254). ALDH1A3 expression in wild-type (WT) and ALDH1A3 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALDH1A3 antibody (Cat#71254, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

 


 

 

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