Description
| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | zinc finger C3HC-type containing 1 |
| Gene Symbol: | ZC3HC1 |
| Synonyms: | NIPA; HSPC216 |
| Gene ID: | 51530 |
| UniProt ID: | Q86WB0 |
| Immunogen: | Recombinant protein of human ZC3HC1 |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis.
Images
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Western blotting analysis using anti-ZC3HC1 antibody (Cat#65310). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZC3HC1 antibody (Cat#65310, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-ZC3HC1 antibody (Cat#65310). ZC3HC1 expression in wild-type (WT) and ZC3HC1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ZC3HC1 antibody (Cat#65310, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of ZC3HC1 expression in HeLa cells using anti-ZC3HC1 antibody (Cat#65310, 1:2,000). Green, isotype control; red, ZC3HC1. |
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