Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Images

	Western blotting analysis using anti-ubiquitin specific peptidase 14 antibody (Cat#62631). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitin specific peptidase 14 antibody (Cat#62631, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Ubiquitin specific peptidase 14 antibody (Cat#62631). Ubiquitin specific peptidase 14 expression in wild type (WT) and Ubiquitin specific peptidase 14 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Ubiquitin specific peptidase 14 expression in HepG2 cells using anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:2,000). Green, isotype control; red, Ubiquitin specific peptidase 14.
	Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 14 antibody (Cat#62631, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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