Description
| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquinol-cytochrome c reductase core protein 2 |
| Gene Symbol: | UQCRC2 |
| Synonyms: | QCR2; UQCR2; MC3DN5 |
| Gene ID: | 7385 |
| UniProt ID: | P22695 |
| Clone ID: | 24GB2220 |
| Immunogen: | A synthesized peptide derived from human UQCRC2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5.
Images
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Western blotting analysis using anti-UQCRC2 antibody (Cat#62633). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UQCRC2 antibody (Cat#62633, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-UQCRC2 antibody (Cat#62633). UQCRC2 expression in wild type (WT) and UQCRC2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UQCRC2 antibody (Cat#62633, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of UQCRC2 expression in HepG2 cells using anti-UQCRC2 antibody (Cat#62633, 1:2,000). Green, isotype control; red, UQCRC2. |
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Immunocytochemical staining of HepG2 cells with anti-UQCRC2 antibody (Cat#62633, 1:1,000). Nuclei were stained blue with DAPI; UQCRC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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