Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin fold modifier 1 |
| Gene Symbol: | UFM1 |
| Synonyms: | HLD14; BM-002; C13orf20 |
| Gene ID: | 51569 |
| UniProt ID: | P61960 |
| Clone ID: | 25GB85 |
| Immunogen: | A synthesized peptide derived from human UFM1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Dec 2008].
Images
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Western blotting analysis using anti-UFM1 antibody (Cat#65192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UFM1 antibody (Cat#65192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-UFM1 antibody (Cat#65192). UFM1 expression in wild-type (WT) and UFM1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UFM1 antibody (Cat#65192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of UFM1 expression in C2C12 cells using anti-UFM1 antibody (Cat#65192, 1:2,000). Green, isotype control; red, UFM1. |
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Immunocytochemical staining of C2C12 cells with anti-UFM1 antibody (Cat#65192, 1:1,000). Nuclei were stained blue with DAPI; UFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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