Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin C-terminal hydrolase L1 |
| Gene Symbol: | UCHL1 |
| Synonyms: | NDGOA; PARK5; PGP95; SPG79; PGP9.5; SPG79A; UCHL-1; Uch-L1; HEL-117; PGP 9.5; HEL-S-53 |
| Gene ID: | 7345 |
| UniProt ID: | P09936 |
| Clone ID: | 23GB2125 |
| Immunogen: | A synthesized peptide derived from human PGP9.5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Images
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Western blotting analysis using anti-UCHL1 antibody (Cat#61188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UCHL1 antibody (Cat#61188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). UCHL1, ubiquitin C-terminal hydrolase L1. |
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Western blotting analysis using anti-UCHL1 antibody (Cat#61188). UCHL1 expression in wild-type (WT) and UCHL1 shRNA knockdown (KD) HAP1 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-UCHL1 antibody (Cat#61188, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of UCHL1 expression in HAP-1 cells using UCHL1 antibody (Cat#61188, 1:2,000). Green, isotype control; red, UCHL1. |
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Immunocytochemical staining of HAP-1 cells with UCHL1 antibody (Cat#61188, 1:1,000). Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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