Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin conjugating enzyme E2 Z |
| Gene Symbol: | UBE2Z |
| Synonyms: | USE1; HOYS7 |
| Gene ID: | 65264 |
| UniProt ID: | Q9H832 |
| Clone ID: | 24GB14295 |
| Immunogen: | Recombinant protein of human UBE2Z |
| Dilution: | WB 1:400-1:2,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis.
Images
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Western blotting analysis using anti-UBE2Z antibody (Cat#65096). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2Z antibody (Cat#65096, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-UBE2Z antibody (Cat#65096). UBE2Z expression in wild-type (WT) and UBE2Z knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBE2Z antibody (Cat#65096, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of UBE2Z expression in HepG2 cells using anti-UBE2Z antibody (Cat#65096, 1:2,000). Green, isotype control; red, UBE2Z. |
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