Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal endoplasmic reticulum (ER)-signal sequence, three catalytically active thioredoxin domains and a C-terminal ER-retention sequence. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S5 gene.

Images

	Western blotting analysis using anti-thioredoxin domain containing 5 antibody (Cat#61261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-thioredoxin domain containing 5 antibody (Cat#61261, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-thioredoxin domain containing 5 antibody (Cat#61261). Thioredoxin domain containing 5 expression in wild type (WT) and thioredoxin domain containing 5 (TXNDC5) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-thioredoxin domain containing 5 antibody (Cat#61261, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Thioredoxin domain containing 5 expression in HT-1080 cells using anti-Thioredoxin domain containing 5 antibody (Cat# 61261, 1:2,000). Green, isotype control; red, Thioredoxin domain containing 5.

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