Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | taxilin alpha |
| Gene Symbol: | TXLNA |
| Synonyms: | IL14; TXLN; IL-14 |
| Gene ID: | 200081 |
| UniProt ID: | P40222 |
| Immunogen: | Recombinant protein of human TXLNA |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable syntaxin binding activity. Predicted to be involved in exocytosis. Predicted to act upstream of or within B cell activation. Located in cytoplasm.
Images
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Western blotting analysis using anti-taxilin alpha antibody (Cat#65304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-taxilin alpha antibody (Cat#65304, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-taxilin alpha antibody (Cat#65304). Taxilin alpha expression in wild-type (WT) and taxilin alpha (TXLNA) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-taxilin alpha antibody (Cat#65304, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Taxilin alpha expression in HAP-1 cells using anti-Taxilin alpha antibody (Cat#65304, 1:2,000). Green, isotype control; red, Taxilin alpha. |
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Immunocytochemical staining of HAP-1 cells with anti-Taxilin alpha antibody (Cat#65304, 1:1,000). Nuclei were stained blue with DAPI; Taxilin alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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