Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Images

	Western blotting analysis using anti-DNA topoisomerase II alpha antibody (Cat#62625). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA topoisomerase II alpha antibody (Cat#62625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-DNA topoisomerase II alpha antibody (Cat#62625). DNA topoisomerase II alpha expression in wild type (WT) and DNA topoisomerase II alpha shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-DNA topoisomerase II alpha antibody (Cat#62625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of DNA topoisomerase II alpha expression in HepG2 cells using anti-DNA topoisomerase II alpha antibody (Cat#62625, 1:2,000). Green, isotype control; red, DNA topoisomerase II alpha.
	Immunocytochemical staining of HepG2 cells with anti-DNA topoisomerase II alpha antibody (Cat#62625, 1:1,000). Nuclei were stained blue with DAPI; DNA topoisomerase II alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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