Background

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two pseudogenes, one on chromosome 15 and the other on chromosome 21, have been found for this gene.

Images

	Western blotting analysis using anti-SRSF9 antibody (Cat#63578). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SRSF9 antibody (Cat#63578, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-SRSF9 antibody (Cat#63578). SRSF9 expression in wild type (WT) and SRSF9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SRSF9 antibody (Cat#63578, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of SRSF9 expression in HeLa cells using anti-SRSF9 antibody (Cat#63578, 1:2,000). Green, isotype control; red, SRSF9.
	Immunocytochemical staining of HeLa cells with anti-SRSF9 antibody (Cat#63578, 1:1,000). Nuclei were stained blue with DAPI; SRSF9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Scale bar, 20 μm.

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