Background

The gene SQSTM1 encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Images

	Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#61668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#61668, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-p62/SQSTM1 antibody (Cat#61668). p62/SQSTM1 expression in wild type (WT) and SQSTM1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-p62/SQSTM1 antibody (Cat#61668, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of p62/SQSTM1 expression in HepG2 cells using anti-p62/SQSTM1 antibody (Cat#61668, 1:2,000). Green, isotype control; red, p62/SQSTM1.
	Immunocytochemical staining of HepG2 cells with anti-p62/SQSTM1 antibody (Cat#61668, 1:1,000). Nuclei were stained blue with DAPI; p62/SQSTM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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