Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4 |
| Gene Symbol: | SMARCA4 |
| Synonyms: | BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; OTSC12; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta |
| Gene ID: | 6597 |
| UniProt ID: | P51532 |
| Clone ID: | 24GB2065 |
| Immunogen: | A synthesized peptide derived from human BRG1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
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Western blotting analysis using anti-SMARCA4 antibody (Cat#62628). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMARCA4 antibody (Cat#62628, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-SMARCA4 antibody (Cat#62628). SMARCA4 expression in wild type (WT) and SMARCA4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMARCA4 antibody (Cat#62628, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of SMARCA4 expression in HepG2 cells using anti-SMARCA4 antibody (Cat#62628, 1:2,000). Green, isotype control; red, SMARCA4. |
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Immunocytochemical staining of HepG2 cells with anti-SMARCA4 antibody (Cat#62628, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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