Description
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | sirtuin 5 |
| Gene Symbol: | SIRT5 |
| Synonyms: | SIR2L5 |
| Gene ID: | 23408 |
| UniProt ID: | Q9NXA8 |
| Clone ID: | 24GB13235 |
| Immunogen: | Recombinant protein of human SIRT5 |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
Images
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Western blotting analysis using anti-sirtuin 5 antibody (Cat#64846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sirtuin 5 antibody (Cat#64846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-sirtuin 5 antibody (Cat#64846). Sirtuin 5 expression in wild-type (WT) and sirtuin 5 (SIRT5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-sirtuin 5 antibody (Cat#64846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using anti-Sirtuin 5 antibody (Cat#64846, 1:2,000). Green, isotype control; red, Sirtuin 5. |
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Immunocytochemical staining of HepG2 cells with anti-Sirtuin 5 associated protein antibody (Cat#64846, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 5 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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