Background

Enables DNA binding activity; histone deacetylase binding activity; and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome; positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway; and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin; nuclear matrix; and paraspeckles.

Images

	Western blotting analysis using anti-SFPQ antibody (Cat#62518). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-SFPQ antibody (Cat#62518). SFPQ expression in wild type (WT) and SFPQ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SFPQ antibody (Cat#62518, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody (Cat#62518, 1:2,000). Green, isotype control; red, SFPQ.
	Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody (Cat#62518, 1:1,000). Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

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