Background

The protein SERPINB1 encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants.

Images

	Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-serpin family B member 1 antibody (Cat#63564). Serpin family B member 1 expression in wild type (WT) and serpin family B member 1 (SERPINB1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-serpin family B member 1 antibody (Cat#63564, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Flow cytometric analysis of Serpin family B member 1 expression in HepG2 cells using anti-Serpin family B member 1 antibody (Cat#63564, 1:2,000). Green, isotype control; red, Serpin family B member 1.

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