Description
| Reactivity: | Human, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Scavenger receptor class B member 1 |
| Gene Symbol: | SCARB1 |
| Synonyms: | CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLCQ6; HDLQTL6 |
| Gene ID: | 949 |
| UniProt ID: | Q8WTV0 |
| Clone ID: | 24GB1395 |
| Immunogen: | A synthesized peptide derived from human Scavenging Receptor SR-BI |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by the gene SCARB1 is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2 and facilitates cell entry by the virus, SARS-CoV2.
Images
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Western blotting analysis using anti-SCARB1 antibody (Cat#62558). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SCARB1 antibody (Cat#62558, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-SCARB1 antibody (Cat#62558). SCARB1 expression in wild type (WT) andSCARB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-SCARB1 antibody (Cat#62558, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Immunocytochemical staining of HepG2 cells with anti-SCARB1 antibody (Cat#62558, 1:1,000). Nuclei were stained blue with DAPI; SCARB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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