Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Images

	Western blotting analysis using anti-Ribophorin I antibody (Cat#62490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ribophorin I antibody (Cat#62490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Ribophorin I antibody (Cat#62490). Ribophorin I expression in wild type (WT) and Ribophorin I shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Ribophorin I antibody (Cat#62490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Ribophorin I expression in HT-1080 cells using anti-Ribophorin I antibody (Cat#62490, 1:2,000). Green, isotype control; red, Ribophorin I.

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