Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | ring finger protein 31 |
| Gene Symbol: | RNF31 |
| Synonyms: | HOIP; Paul; ZIBRA; IMD115 |
| Gene ID: | 55072 |
| UniProt ID: | Q96EP0 |
| Immunogen: | Recombinant protein of human RNF31 |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.
Images
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Western blotting analysis using anti-RNF31 antibody (Cat#65166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-RNF31 antibody (Cat#65166). RNF31 expression in wild-type (WT) and RNF31 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RNF31 antibody (Cat#65166, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of RNF31 expression in HT-1080 cells using anti-RNF31 antibody (Cat#65166, 1:2,000). Green, isotype control; red, RNF31. |
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Immunocytochemical staining of HT-1080 cells with anti-RNF31 antibody (Cat#65166, 1:1,000). Nuclei were stained blue with DAPI; RNF31 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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