Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | retinol dehydrogenase 14 |
| Gene Symbol: | RDH14 |
| Synonyms: | PAN2; SDR7C4 |
| Gene ID: | 57665 |
| UniProt ID: | Q9HBH5 |
| Clone ID: | 24GB13960 |
| Immunogen: | Recombinant protein of human RDH14 |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables all-trans-retinol dehydrogenase (NADP+) activity. Involved in osteoblast differentiation. Located in cytosol; endoplasmic reticulum; and nucleoplasm.
Images
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Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Retinol dehydrogenase 14 expression in wild-type (WT) and retinol dehydrogenase 14 (RDH14) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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