Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | regulator of chromosome condensation 1 |
| Gene Symbol: | RCC1 |
| Synonyms: | CHC1; RCC1-I |
| Gene ID: | 1104 |
| UniProt ID: | P18754 |
| Clone ID: | 25GB3350 |
| Immunogen: | A synthesized peptide derived from human RCC1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables several functions, including guanyl-nucleotide exchange factor activity; nucleosomal DNA binding activity; and protein heterodimerization activity. Involved in G1/S transition of mitotic cell cycle; regulation of mitotic nuclear division; and spindle organization. Located in chromatin; cytoplasm; and nuclear lumen. Part of protein-containing complex.
Images
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Western blotting analysis using anti-regulator of chromosome condensation 1 antibody (Cat#61694). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-regulator of chromosome condensation 1 antibody (Cat#61694, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-regulator of chromosome condensation 1 antibody (Cat#61694). Regulator of chromosome condensation 1 expression in wild type (WT) and regulator of chromosome condensation 1 (RCC1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-regulator of chromosome condensation 1 antibody (Cat#61694, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of Regulator of chromosome condensation 1 expression in HepG2 cells using anti-Regulator of chromosome condensation 1 antibody (Cat#61694, 1:2,000). Green, isotype control; red, Regulator of chromosome condensation 1. |
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