Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAB5C, member RAS oncogene family |
| Gene Symbol: | RAB5C |
| Synonyms: | RABL; L1880; RAB5L; RAB5CL |
| Gene ID: | 5878 |
| UniProt ID: | P51148 |
| Clone ID: | 25GB3190 |
| Immunogen: | A synthesized peptide derived from human RAB5C / RABL |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
Images
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Western blotting analysis using anti-RAB5C antibody (Cat#61660). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-RAB5C antibody (Cat#61660). RAB5C expression in wild type (WT) and RAB5C knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAB5C antibody (Cat#61660, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of RAB5C expression in HepG2 cells using anti-RAB5C antibody (Cat#61660, 1:2,000). Green, isotype control; red, RAB5C. |
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Immunocytochemical staining of HepG2 cells with anti-RAB5C antibody (Cat#61660, 1:1,000). Nuclei were stained blue with DAPI; RAB5C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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