Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

Images

	Western blotting analysis using anti-PTPN11 antibody (Cat#63323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus ECL Super Kit (Cat#636). PTPN11, protein tyrosine phosphatase non-receptor type 11.
	Western blotting analysis using anti-PTPN11 antibody (Cat#63323). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of PTPN11 expression in HepG2 cells using anti-PTPN11 antibody (Cat#63323, 1:2,000). Green, isotype control; red,PTPN11.
	Immunocytochemical staining of HepG2 cells with anti-PTPN11 protein antibody (Cat#63323, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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