Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#61560). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#61560, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-protein tyrosine kinase 2 antibody (Cat#61560). Protein tyrosine kinase 2 expression in wild-type (WT) and protein tyrosine kinase 2 (PTK2) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein tyrosine kinase 2 antibody (Cat#61560, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Protein tyrosine kinase 2 expression in C2C12 cells using anti-Protein tyrosine kinase 2 antibody (Cat#61560, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2.
	lmmunocytochemical staining of C2C12 cells with Protein tyrosine kinase 2 antibody (Cat#61560, 1:1,000). Nuclei were stained blue with DAPl; Protein tyrosine kinase 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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