Background

This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.

Images

	Western blotting analysis using anti-Prostaglandin E synthase 3 antibody (Cat#62574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Prostaglandin E synthase 3 antibody (Cat#62574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-prostaglandin E synthase 3 antibody (Cat#62574). Prostaglandin E synthase 3 expression in wild-type (WT) and prostaglandin E synthase 3 (PTGES3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-prostaglandin E synthase 3 antibody (Cat#62574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Prostaglandin E synthase 3 expression in HepG2 cells using anti-Prostaglandin E synthase 3 antibody (Cat#62574, 1:2,000). Green, isotype control; red, Prostaglandin E synthase 3.

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