Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | PC4 and SRSF1 interacting protein 1 |
| Gene Symbol: | PSIP1 |
| Synonyms: | p52; p75; PAIP; DFS70; LEDGF; PSIP2 |
| Gene ID: | 11168 |
| UniProt ID: | O75475 |
| Clone ID: | 24GB840 |
| Immunogen: | A synthesized peptide derived from human PSIP1 / LEDGF |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables DNA-binding transcription factor binding activity; chromatin binding activity; and transcription coactivator activity. Involved in mRNA 5′-splice site recognition and positive regulation of transcription by RNA polymerase II. Located in heterochromatin; nuclear periphery; and nucleoplasm.
Images
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Western blotting analysis using anti-PSIP1 antibody (Cat#62438). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSIP1 antibody (Cat#62438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-PSIP1 antibody (Cat#62438). PSIP1 expression in wild-type (WT) and PSIP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PSIP1 antibody (Cat#62438, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of PSIP1 expression in HeLa cells using PSIP1 antibody (Cat#62438, 1:2,000). Green, isotype control; red, PSIP1. |
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Immunocytochemical staining of Hela cells with anti-PSIP1 antibody (Cat#62438, 1:1,000). Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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