Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | pre-mRNA splicing tri-snRNP complex factor PRPF4 |
| Gene Symbol: | PRPF4 |
| Synonyms: | PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60 |
| Gene ID: | 9128 |
| UniProt ID: | O43172 |
| Clone ID: | 24GB975 |
| Immunogen: | A synthesized peptide derived from human PRPF4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.
Images
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Western blotting analysis using anti-PRPF4 antibody (Cat#62466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRPF4 antibody (Cat#62466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-PRPF4 antibody (Cat#62466). PRPF4 expression in wild type (WT) and PRPF4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PRPF4 antibody (Cat#62466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of PRPF4 expression in HepG2 cells using anti-PRPF4 antibody (Cat#62466, 1:2,000). Green, isotype control; red, PRPF4. |
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Immunocytochemical staining of HepG2 cells with anti-PRPF4 antibody (Cat#62466, 1:1,000). Nuclei were stained blue with DAPI; PRPF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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