Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phospholipid scramblase 3 |
| Gene Symbol: | PLSCR3 |
| Gene ID: | 57048 |
| UniProt ID: | Q9NRY6 |
| Clone ID: | 24GB1345 |
| Immunogen: | A synthesized peptide derived from human PLSCR3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables calcium-dependent protein binding activity; metal ion binding activity; and phospholipid scramblase activity. Involved in several processes, including apoptotic process; cardiolipin biosynthetic process; and regulation of release of cytochrome c from mitochondria. Located in cytosol; mitochondrion; and plasma membrane.
Images
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Western blotting analysis using anti-Phospholipid scramblase 3 antibody (Cat#62545). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospholipid scramblase 3 antibody (Cat#62545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-Phospholipid scramblase 3 antibody (Cat#62545). Phospholipid scramblase 3 expression in wild type (WT) and Phospholipid scramblase 3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Phospholipid scramblase 3 antibody (Cat#62545, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Phospholipid scramblase 3 expression in H9c2 cells using anti-Phospholipid scramblase 3 antibody (Cat#62545, 1:2,000). Green, isotype control; red, Phospholipid scramblase 3. |
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