Background

The protein encoded by the gene MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Images

	Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-Phospho-mTOR (S2481) antibody (Cat#62329). Phospho-mTOR (S2481) expression in wild type (WT) and Phospho-mTOR (S2481) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Phospho-mTOR (S2481) expression in HepG2 cells using Phospho-mTOR (S2481) antibody (Cat#62329, 1:2,000). Green, isotype control; red, Phospho-mTOR (S2481).
	Immunocytochemical staining of HepG2 cells with anti-Phospho-mTOR (S2481) antibody (Cat#62329, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2481) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

You may also be interested in: