Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene.

Images

	Western blotting analysis using anti-phosphogluconate dehydrogenase antibody (Cat#61656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phosphogluconate dehydrogenase antibody (Cat#61656, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-phosphogluconate dehydrogenase antibody (Cat#61656). Phosphogluconate dehydrogenase expression in wild type (WT) and phosphogluconate dehydrogenase (PGD) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phosphogluconate dehydrogenase antibody (Cat#61656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Phosphogluconate dehydrogenase expression in HepG2 cells using anti-Phosphogluconate dehydrogenase antibody (Cat#61656, 1:2,000). Green, isotype control; red, Phosphogluconate dehydrogenase.
	lmmunocytochemical staining of HepG2 cells with Phosphogluconate dehydrogenase antibody (Cat#61656, 1:1,000). Nuclei were stained blue with DAPl; Phosphogluconate dehydrogenase was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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