Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | penta-EF-hand domain containing 1 |
| Gene Symbol: | PEF1 |
| Synonyms: | ABP32; PEF1A |
| Gene ID: | 553115 |
| UniProt ID: | Q9UBV8 |
| Clone ID: | 24GB1340 |
| Immunogen: | A synthesized peptide derived from human PEF1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.
Images
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Western blotting analysis using anti-PEF1 antibody (Cat#62544). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PEF1 antibody (Cat#62544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-PEF1 antibody (Cat#62544). PEF1 expression in wild type (WT) and PEF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-PEF1 antibody (Cat#62544, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of PEF1 expression in Hela cells using anti-PEF1 antibody (Cat#62544, 1:2,000). Green, isotype control; red, PEF1. |
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