Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein kinase C and casein kinase substrate in neurons 3 |
| Gene Symbol: | PACSIN3 |
| Synonyms: | SDPIII |
| Gene ID: | 29763 |
| UniProt ID: | Q9UKS6 |
| Clone ID: | 25GB505 |
| Immunogen: | Recombinant protein of human PACSIN3 |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants.
Images
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Western blotting analysis using anti-PACSIN3 antibody (Cat#61259). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PACSIN3 antibody (Cat#61259, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-PACSIN3 antibody (Cat#61259). PACSIN3 expression in wild type (WT) and PACSIN3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PACSIN3 antibody (Cat#61259, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Flow cytometric analysis of PACSIN3 expression in HepG2 cells using anti-PACSIN3 antibody (Cat# 61259, 1:2,000). Green, isotype control; red, PACSIN3. |
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