Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | olfactomedin 4 |
| Gene Symbol: | OLFM4 |
| Synonyms: | GC1; OLM4; OlfD; pDP4; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1 |
| Gene ID: | 10562 |
| UniProt ID: | Q6UX06 |
| Clone ID: | 24GB680 |
| Immunogen: | A synthesized peptide derived from human OLFM4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.
Images
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Western blotting analysis using anti-Olfactomedin 4 antibody (Cat#62403). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Olfactomedin 4 antibody (Cat#62403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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Western blotting analysis using anti-Olfactomedin 4 antibody (Cat#62403). Olfactomedin 4 expression in wild type (WT) and Olfactomedin 4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Olfactomedin 4 antibody (Cat#62403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of Olfactomedin 4 expression in C2C12 cells using Olfactomedin 4 antibody (Cat#62403, 1:2,000). Green, isotype control; red, Olfactomedin 4. |
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Immunocytochemical staining of C2C12 cells with anti-Olfactomedin 4 antibody (Cat#62403, 1:1,000). Nuclei were stained blue with DAPI; Olfactomedin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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