Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NPC intracellular cholesterol transporter 2 |
| Gene Symbol: | NPC2 |
| Synonyms: | HE1; EDDM1 |
| Gene ID: | 10577 |
| UniProt ID: | P61916 |
| Clone ID: | 24GB13695 |
| Immunogen: | Recombinant protein of human Niemann Pick C2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.
Images
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Western blotting analysis using anti-NPC2 antibody (Cat#64907). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NPC2 antibody (Cat#64907, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-NPC2 antibody (Cat#64907). NPC2 expression in wild-type (WT) and NPC2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NPC2 antibody (Cat#64907, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of NPC2 expression in C2C12 cells using anti-NPC2 antibody (Cat#64907, 1:1,000). Green, isotype control; red, NPC2. |
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Immunocytochemical staining of C2C12 cells with anti-NPC2 antibody (Cat#64907, 1:1,000). Nuclei were stained blue with DAPI; NPC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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