Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Images

	Western blotting analysis using anti-neurofilament medium chain antibody (Cat#63480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
	Western blotting analysis using anti-neurofilament medium chain antibody (Cat#63480). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of Neurofilament medium chain expression in HeLa cells using anti-Neurofilament medium chain antibody (Cat#63480, 1:2,000). Green, isotype control; red, Neurofilament medium chain.

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