Description
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mitotic arrest deficient 1 like 1 |
| Gene Symbol: | MAD1L1 |
| Synonyms: | MAD1; MVA7; PIG9; TP53I9; TXBP181 |
| Gene ID: | 8379 |
| UniProt ID: | Q9Y6D9 |
| Clone ID: | 25GB3060 |
| Immunogen: | A synthesized peptide derived from human MAD1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Images
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Western blotting analysis using anti-MAD1L1 antibody (Cat#61622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD1L1 antibody (Cat#61622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Western blotting analysis using anti-MAD1L1 antibody (Cat#61622). MAD1L1 expression in wild-type (WT) and MAD1L1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD1L1 antibody (Cat#61622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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Flow cytometric analysis of MAD1L1 expression in HepG2 cells using anti-MAD1L1 antibody (Cat#61622, 1:2,000). Green, isotype control; red, MAD1L1. |
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Immunocytochemical staining of HepG2 cells with anti-MAD1L1 antibody (Cat#61622, 1:1,000). Nuclei were stained blue with DAPI; MAD1L1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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