Name: KD-Validated MACROH2A1 Rabbit mAb (20 μl)
Brand: Genzera
Price: 149 USD
Availability: InStock

Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	macroH2A.1 histone
Gene Symbol:	MACROH2A1
Synonyms:	H2A.y; H2A/y; H2AFY; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2
Gene ID:	9555
UniProt ID:	O75367
Clone ID:	25GB485
Immunogen:	A synthesized peptide derived from human macroH2A.1
Dilution:	WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:	Affinity purified
Concentration:	Lot dependent
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Images

	Western blotting analysis using anti-MacroH2A.1 Histone antibody (Cat#61249). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MacroH2A.1 Histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Western blotting analysis using anti-macroH2A.1 histone antibody (Cat#61249). MacroH2A.1 histone expression in wild type (WT) and macroH2A.1 histone (H2AFY) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-macroH2A.1 histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
	Flow cytometric analysis of MacroH2A.1 Histone expression in HAP-1 cells usiMacroH2A.1 Histone0 α antibody (Cat# 61249, 1:2,000). Green, isotype control; red, MacroH2A.1 Histone.